ev d68 strains us mo  (ATCC)

Journal: bioRxiv

Article Title: Paramyxovirus matrix proteins modulate host cell translation via exon-junction complex interactions in the cytoplasm

doi: 10.1101/2024.09.05.611502

Figure Lengend Snippet: HEK-293T cells were transfected with siRNA pools targeting eIF4A3, Y14, MAGOH, or non-targeting control siRNAs (NC), respectively. At 48 hrs post-transfection, cells were inoculated with the designated virus (A) HPIV3, (B) Cedar, (C) MuV, (D) NDV, (E) Influenza A (A/WSN/1933), (F) Enterovirus D68, and (G) SARA-CoV2 at a multiplicity of infection (m.o.i.) of 0.01. The titers of infectious supernatants were determined on Vero-CCL81 cells using a 10-fold serial dilution at the indicated time points. For each virus, the expression levels of endogenous eIF4AIII, Y14, and MAGOH, along with infection control for viral protein or EGFP reporter, were analyzed by immunoblotting; results shown beside each panel confirm the knockdown of target proteins and validate virus infection. Symbols represent the data points from biological triplicates. Bars represent the mean of the triplicates. Statistical significance was determined by two-way ANOVA with Dunnett multiple comparison test. * P <0.05; ** P <0.01; *** P <0.001; **** P <0.0001; ns, not significant. Immunoblottings are shown beside each to determine the knockdown of target proteins and controls for virus infection.

Article Snippet: EV D68 strain US/MO/14-18947 (ATCC, VR-1823) were amplified in RD cells.

Techniques: Transfection, Control, Virus, Infection, Serial Dilution, Expressing, Western Blot, Knockdown, Comparison